Rotation Invariant Clustering of 3D Cell Nuclei Shapes.

Cellular imaging with confocal fluorescence laser microscopy gave rise to many new insights into the cellular machinery. One interesting observation suggests that morphology of cell nucleus plays a key role for neutrophilic function, which is an essential part of the innate immune system of most mammals.
Due to the increasing availability of high resolution 3D images coming from the microscope, machine learning becomes a promising tool for automatically discovering underlying hidden structures. Here, the major difficulty consists of selecting an appropriate representation for characterizing the morphology of cell nucleus.
In this work we tackle this problem and propose a fully unsupervised mechanism for finding structure in high-throughput 3D image data. The key component of our approach is based on Generic Fourier Transform (GFT) for 2D images, which for 3D involves spherical coordinate transformation prior to fast Discrete Fourier Transformation.
On top on GFT we apply dimensionality reduction with Principal Component Analysis, followed by generative cluster analysis with a Gaussian Mixture Model. We validate our new approach first on a synthetic 3D-MNIST dataset with random rotations, where quantitative and qualitative results confirm the applicability of the proposed pipeline for exploring shape space in a purely unsupervised manner.

Slc46a1 (GFP-tagged) - Mouse DNA segment, Chr 11, ERATO Doi 18, expressed (D11Ertd18e)

MG207332 10 µg Ask for price

Ldlrad4 (untagged) - Mouse DNA segment, Chr 18, ERATO Doi 653, expressed (D18Ertd653e), (10ug)

MC209900 10 µg Ask for price

Ldlrad4 (GFP-tagged) - Mouse DNA segment, Chr 18, ERATO Doi 653, expressed (D18Ertd653e)

MG204315 10 µg Ask for price

DiscoveryProbe? DNA Damage/DNA Repair Library

L1033-.1 100 uL/well(10 mM solution)
EUR 4666.8

DiscoveryProbe? DNA Damage/DNA Repair Library

L1033-.25 250 uL/well(10 mM solution)
EUR 8286

DiscoveryProbe? DNA Damage/DNA Repair Library

L1033-5 5 mg/well
EUR 10806

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N150 500 units
EUR 122.4

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N152 5x500 units
EUR 472.8

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N154 20x500 units
EUR 1830

T7 DNA

310-005 200µg
EUR 98.4

T7 DNA

310-025 5x200 µg
EUR 270

T4 DNA

enz-286 20,000IU
EUR 65
Description: Recombinant T4 DNA Ligase

DNA Ligase I (DNA Ligase I) Antibody

abx232431-100ug 100 ug
EUR 577.2

DNA pol

ABF9055 100 ug
EUR 525.6

DNA STAT

TL-4210 100ml
EUR 158

DNA STAT

TL-4220 200ml
EUR 276

DNA PKcs

E8ET1610-12 100ul
EUR 275
Description: Available in various conjugation types.

pUC18 DNA

G40-300 50µg
EUR 67.2

pUC18 DNA

G40-305 5x50µg
EUR 176.4

DNA-Fect

DF37100-1 1 ml
EUR 418.8

pBR322 DNA

MSD14 50ug
EUR 183.19

Lambda DNA

PCR-259 2 x 1ml
EUR 59.1

DNA Primase

E8ER1907-59 100ul
EUR 275
Description: Available in various conjugation types.

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

AD-146-U Custom Ask for price

DNA antibody

10-D20A 500 ug
EUR 591.6
Description: Mouse monoclonal DNA antibody

DNA antibody

10C-CR6034M1 100 ug
EUR 289.2
Description: Mouse monoclonal DNA antibody

DNA antibody

10R-D114b 100 ug
EUR 672
Description: Mouse monoclonal DNA antibody

DNA Antibody

abx023805-02mg 0.2 mg
EUR 777.6

DiscoveryProbe™ DNA Damage/DNA Repair Library

L1033-.096 100uL/well(10 mM solution),96 Well Deep Well Plate
EUR 6980
Description: A unique collection of 481 DNA Damage/DNA repair-related compounds for DNA damage/DNA repair research.

Activated DNA

80605 500 µL
EUR 125
Description: Activated DNA for PARP assay, for use with BPS's assay kits for PARPs 1, 2, 3, and 6. PARP1 Chemiluminescent Assay Kit, #80551 ; PARP2 Chemiluminescent Assay Kit, #80552; PARP3 Chemiluminescent Assay Kit, #80553; PARP6 Chemiluminescent Assay Kit, #80556; PARP2 Homogeneous Assay Kit, #80702

DNA-Fect293

DF37293-1 1 ml
EUR 349.2

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

Ldlrad4 (Myc-DDK-tagged) - Mouse DNA segment, Chr 18, ERATO Doi 653, expressed (D18Ertd653e)

MR204315 10 µg Ask for price

DNA Polymerase

ABD3082 100 ug
EUR 525.6

DNA Polymerase

ABF5235 100 ug
EUR 525.6

DNA-fectamine

DNAF001 1ml
EUR 334.57

DNA-Spermidine

T31554-10mg 10mg Ask for price
Description: DNA-Spermidine

DNA-Spermidine

T31554-1g 1g Ask for price
Description: DNA-Spermidine

DNA-Spermidine

T31554-1mg 1mg Ask for price
Description: DNA-Spermidine

DNA-Spermidine

T31554-50mg 50mg Ask for price
Description: DNA-Spermidine

DNA-Spermidine

T31554-5mg 5mg Ask for price
Description: DNA-Spermidine

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (1mg Relaxed DNA + 2000u Gyrase)

TG2000G-7KIT 2000 assays
EUR 3412.8

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (500ug Relaxed DNA + 1000u Gyrase)

TG2000G-5KIT 1000 assays
EUR 1934.4

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-BP889065HU
  • EUR 1008.00
  • EUR 398.40
  • EUR 2604.00
  • EUR 1362.00
  • EUR 1894.80
  • EUR 564.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Baculovirus

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-YP889065HU
  • EUR 703.20
  • EUR 358.80
  • EUR 2606.40
  • EUR 1080.00
  • EUR 1730.40
  • EUR 458.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Yeast

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-EP889065HU
  • EUR 606.00
  • EUR 318.00
  • EUR 2192.40
  • EUR 919.20
  • EUR 1461.60
  • EUR 402.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in E.coli

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
EUR 60

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N142 5x500 units
EUR 144

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N144 20x500 units
EUR 440.4

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled

AD-146-F Custom Ask for price

Lenti ORF clone of DXBay18 (mGFP-tagged) - Mouse DNA segment, Chr X, Baylor 18 (DXBay18)

MR214768L4 10 µg Ask for price

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (50ug Relaxed DNA + 100u Gyrase)

TG2000G-1KIT 100 assays
EUR 456

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (250ug Relaxed DNA + 500u Gyrase)

TG2000G-3KIT 500 assays
EUR 1182

DNA Amplification Kit with Taq DNA Polymerase, 100app

PL1202-K each Ask for price

DNA nucleotidylexotransferase

AP88338 1mg
EUR 2640

DNA nucleotidylexotransferase

AP88424 1mg
EUR 2640

Human Neutrophil Elastase (DNA I) , DNA Aptamer, unlabeled

AD-155-U Custom Ask for price

Recombinant human DNA-directed DNA/RNA polymerase mu

P2815 100ug Ask for price
Description: Recombinant protein for human DNA-directed DNA/RNA polymerase mu

Human Neutrophil Elastase (DNA I) , DNA Aptamer, Biotinylated

AD-155-B Custom Ask for price

Mouse Anti-DNA Antibody (DNA) ELISA Kit

abx257816-96tests 96 tests
EUR 895.2

DiscoveryProbe? DNA Damage/DNA Repair-related Compounds Panel

L1007-5 5 mg/well
EUR 7172.4
Description: A wide range of well-characterized bioactive molecules that covers various targets related to DNA damage/DNA repair, including ATM/ATR, HDAC, and topoisomerase etc. Facilitate your research towards the insights of cancer, genome instability and immune diseases etc.

pUC 19 DNA

MSD16 50ug
EUR 136.21

pUC 57 DNA

MSD17 50ug
EUR 136.21

DNA Pure Kit

FYG601-100P 100 Preps Ask for price

DNA Pure Kit

FYG601-300P 300 Preps Ask for price

Taq Plus DNA

enz-309 250U
EUR 60
Description: Recombinant Taq Plus DNA Polymerase

Soil DNA Kit

AGDK-0016 Kit Ask for price
Description: Soil DNA Kit offers a rapid and easy-to-use method for the isolation of microbial genomic DNA from all soil samples. This kit is based on spin column chromatography as the separation matrix. After cell lysis, the DNA purification process can be completed in fewer than 20 minutes. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream application such as PCR, qPCR, genotyping, sequencing, Southern Blot analysis, and more.

Soil DNA Kit

AGDK-0035 Kit Ask for price
Description: Soil DNA Kit is designed for isolation of high quality DNA from from soil samples. The kit utilizes our unique high-capacity magnetic bead technology that ensures efficient recovery of DNA fragment library within a desired fragment length range. After cell lysis, the DNA purification process can be completed in fewer than 20 minutes. The purified DNA is completely compatible with downstream application such as PCR, qPCR, genotyping, sequencing, Southern Blot analysis, and more. The system allows for automation after sample lysis via Hamilton STAR™, Thermo KingFisher™ Flex, Applied Biosystems® MagMAX™, Qiagen Biosprint, and other liquid handling instruments.

2K DNA Marker

20-abx098045
  • EUR 309.60
  • EUR 226.80
  • 2.5 ml
  • 500 ul

5K DNA Marker

20-abx098048
  • EUR 309.60
  • EUR 226.80
  • 2.5 ml
  • 500 ul

T4 DNA Ligase

abx098136-10kU 10 kU
EUR 385.2

T4 DNA Ligase

abx098136-20Ku 20 Ku
EUR 477.6

Phage λ-DNA

301-025 500 µg
EUR 122.4

Phage λ-DNA

301-100 5x500µg
EUR 426

T4 DNA ligase

402-002 400 WEISS UNITS
EUR 75.6

T4 DNA ligase

402-010 2000 WEISS UNITS
EUR 184.8

DNA Assay Kit

6023 1 kit
EUR 216.6
Description: DNA Assay Kit

DNA Assay Kit

Z5030023 250 assays
EUR 636

Viral DNA Kit

AGDK-0010 Kit Ask for price
Description: Viral DNA Kit offers a rapid and easy-to-use method for the isolation of high quality virus DNA from 1-200ul serum, plasma, and cell-free medium. This kit is based on spin column chromatography as the separation matrix. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream application such as PCR, qPCR, genotyping, sequencing, Southern Blot analysis, and more.

Plant DNA Kit

AGDK-0011 Kit Ask for price
Description: Plant DNA Kit is designed for isolation of high quality DNA from from a wide variety of plant species. Up to 100 mg of wet samples or 30 mg dry samples can be processed in less than 30 minutes.This kit is based on spin column chromatography as the separation matrix. This kit uses a novel and proprietary method to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant sample.The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream application such as PCR, qPCR, genotyping, sequencing, Southern Blot analysis, and more.

Plant DNA Kit

AGDK-0028 Kit Ask for price
Description: Plant DNA Kit is designed for isolation of high quality DNA from from a wide variety of plant species. Up to 100 mg of wet samples or 30 mg dry samples can be processed in less than 30 minutes. The kit utilizes our unique high-capacity magnetic bead technology that ensures efficient recovery of DNA fragment library within a desired fragment length range. The purified DNA is completely compatible with downstream application such as PCR, qPCR, genotyping, sequencing, Southern Blot analysis, and more.

T4 DNA Ligase

RK21501 80000U
EUR 10

DNA Ligase IV

E8ET1705-77 100ul
EUR 275
Description: Available in various conjugation types.

DNA Ligase I

E8ET7107-14 100ul
EUR 275
Description: Available in various conjugation types.

T4 DNA Ligase

G467 200 U (200 μl)
EUR 60
Description: T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.

T4 DNA Ligase

EN-149L 5 x 400Weiss units
EUR 149.7
Description: E. coli lambda lysogen NM 989

T4 DNA Ligase

EN-149S 400Weiss units
EUR 37.6
Description: E. coli lambda lysogen NM 989

DNA ligase 1

AP82345 1mg
EUR 2640

DNA ligase 3

AP82579 1mg
EUR 2640

DNA ligase 3

AP82720 1mg
EUR 2640

DNA ligase 1

AP82852 1mg
EUR 2640

DNA ligase 1

AP83036 1mg
EUR 2640

15K DNA Marker

20-abx098049
  • EUR 326.40
  • EUR 226.80
  • 2.5 ml
  • 500 ul

1Kb DNA Marker

20-abx098050
  • EUR 309.60
  • EUR 226.80
  • 2.5 ml
  • 500 ul

1Kb DNA Marker

D1101-050 50㎍
EUR 157.2

1Kb DNA Marker

D1101-250 5X50㎍
EUR 360

SYBR DNA Stain

PCR-273 5 x 1,8ml
EUR 48.2

Taq DNA Ligase

RK20502 2000U
EUR 105

DNA LADDER (1KB)

IB01351 50uG/500uL
EUR 61.65
Description: [FR]

Genomic DNA Kit

20-abx098076
  • EUR 526.80
  • EUR 292.80
  • 200 rxns
  • 50 rxns

DNA PK Antibody

abx149863-100ug 100 ug
EUR 526.8

DNA PK antibody

20R-1466 100 ug
EUR 807.6
Description: Rabbit polyclonal DNA PK antibody

DNA- PK Antibody

ABF0550 100 ug
EUR 525.6

DNA PK Antibody

ABF5340 100 ug
EUR 525.6

DNA Loading Dye

G030 6X; 3 x 1.0 ml
EUR 94.8

Calf thymus DNA

HY-109517 100mg
EUR 2292

Calf thymus DNA

T13592-10mg 10mg Ask for price
Description: Calf thymus DNA

Calf thymus DNA

T13592-1g 1g Ask for price
Description: Calf thymus DNA

Calf thymus DNA

T13592-1mg 1mg Ask for price
Description: Calf thymus DNA

Calf thymus DNA

T13592-50mg 50mg Ask for price
Description: Calf thymus DNA

Calf thymus DNA

T13592-5mg 5mg Ask for price
Description: Calf thymus DNA

DNA PK Antibody

AF5340 100ul
EUR 280
Description: Human,Mouse

E.coli DNA Ligase

RK20501 1000U
EUR 20.5

DNA LADDER (100bp)

IB01301 50uG/500uL
EUR 59.65
Description: [FR]

DNA LADDER (100bp)

IB01302 50uG/500uL
EUR 56.1
Description: (NO LOADING DYE) [FR]

GENOMIC DNA KIT

IB47250 2 X 96 PREP KIT
EUR 759.68

GENOMIC DNA KIT

IB47251 4 X 96 PREP KIT
EUR 1464.04

GENOMIC DNA KIT

IB47252 10 X 96 PREP KIT
EUR 3254.61

DNA PK Antibody

AF5340-100ul 100ul
EUR 280

DNA PK Antibody

AF5340-200ul 200ul
EUR 350

DNA PK Antibody

E18-5340-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA PK Antibody

E18-5340-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Fig Genomic DNA

PLG-1042 0.1mg
EUR 307

Oat Genomic DNA

PLG-1096 0.1mg
EUR 307

Rye Genomic DNA

PLG-1097 0.1mg
EUR 307

Pea Genomic DNA

PLG-1141 0.1mg
EUR 307

ELK Genomic DNA

GE-240 0.1mg
EUR 177

Cat Genomic DNA

GC-130 0.1mg
EUR 177

DNA Amplification Kit with Max Taq DNA Polymerase, 100app

PL2202-K each Ask for price

100bp DNA Marker

20-abx098052
  • EUR 326.40
  • EUR 226.80
  • 2.5 ml
  • 500 ul

100bp DNA ladder

9K-004-0002 0.5ml
EUR 260.98

100bp DNA Marker

D1100-050 50㎍
EUR 198

100bp DNA Marker

D1100-250 5X50㎍
EUR 660

Pork Genomic DNA

PCR-705 20µg
EUR 90.1

100bp DNA ladder

TBS4030-0.5mL 0.5 ml
EUR 60

100bp DNA ladder

TBS4030-1.0mL 1.0 ml
EUR 110

DNA pol Antibody

E18-9055-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

DNA pol Antibody

E18-9055-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

DNA-PKcs/PRKDC

E8M1204-6 100ul
EUR 275
Description: Available in various conjugation types.

Corn Genomic DNA

PLG-1002 0.1mg
EUR 307

Rice Genomic DNA

PLG-1004 0.1mg
EUR 307

Pear Genomic DNA

PLG-1033 0.1mg
EUR 307

Duck Genomic DNA

GD-220 0.1mg
EUR 177

Goat Genomic DNA

GG-150 0.1mg
EUR 177

Crab Genomic DNA

GRA-340 0.025mg
EUR 177

Clam Genomic DNA

GCL-325 0.025mg
EUR 177

Human Neutrophil Elastase (DNA I) , DNA Aptamer, FITC labeled

AD-155-F Custom Ask for price

DNA PKcs antibody

10R-10729 100 ug
EUR 457.2
Description: Mouse monoclonal DNA PKcs antibody

DNA PKcs antibody

20R-2017 50 ug
EUR 337.2
Description: Rabbit polyclonal DNA PKcs antibody

DNA PKcs antibody

20R-2346 50 ug
EUR 337.2
Description: Rabbit polyclonal DNA PKcs antibody

DNA-PK Antibody

3130-100 each
EUR 405.6

Human Genomic DNA

BIO-35025 500µl @ 200ng/µl Ask for price

DNA PKcs antibody

70R-50261 100 ul
EUR 292.8
Description: Purified Polyclonal DNA PKcs antibody

DNA-PK Antibody

33358-100ul 100ul
EUR 302.4

DNA-PK Antibody

33358-50ul 50ul
EUR 224.4

DNA PKcs Antibody

48923-100ul 100ul
EUR 399.6

DNA PKcs Antibody

48923-50ul 50ul
EUR 286.8

T4 DNA Polymerase

N101-01 2000 U
EUR 278.4

T4 DNA Polymerase

M1211-100 each
EUR 385.2

DL5000 DNA Marker

MD102-01 250 μl
EUR 128.4

DL5000 DNA Marker

MD102-02 500 μl
EUR 135.6

Human Genomic DNA

PCR-261 20µg
EUR 96

Horse Genomic DNA

PCR-706 20µg
EUR 90.1

Human Genomic DNA 

X11000
  • Ask for price
  • EUR 235.40
  • 0.2 ml
  • 0.2 ml

DNA-PK Antibody

AF6631 100ul
EUR 280
Description: Human,Mouse

DNA-PK Antibody

AF0550 100ul
EUR 280
Description: Human,Mouse

DNA-PK Antibody

8C0174 50ug
EUR 368
Description: DNA-PK Antibody

DNA-PK Antibody

E11-0174C 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA-PK Antibody

E11-0421B 100μg
EUR 225
Description: Available in various conjugation types.

DNA-PK Antibody

E11-0906B 100μg
EUR 225
Description: Available in various conjugation types.
Then we apply our proposed technique to a new collected dataset of high resolution 3D images of neutrophile nuclei suggesting a clustering model with six significant clusters of morphological cell nuclei prototypes. We visualize differences in the cell shape clusters by providing prototypical examples of neutrophilic cell nuclei.