Bridging-induced microphase separation: photobleaching experiments, chromatin domains and the need for active reactions.
We review the mechanism and consequences of the ‘bridging-induced attraction’, a generic biophysical principle that underpins some existing models for chromosome organization in 3D.
This attraction, which was revealed in polymer physics-inspired computer simulations, is a generic clustering tendency arising in multivalent chromatin-binding proteins, and it provides an explanation for the biogenesis of nuclear bodies and transcription factories via microphase separation.
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| ZTF-100-L-R-S | CORNING | 960/pk | EUR 478 |
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| 4073-STD | CORNING | 1/pk | EUR 203 |
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Description: Corning and Axygen Liquid Handling Equipment; Corning Lambda Plus Single Channel Pipettors |
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 100UL AXYGEN MULTIRACK FILTERED TIP, RACKED, STERILE, 960/PCK 5 PCK/CASE |
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| MRF-100-R-S | CORNING | 960/pk | EUR 327 |
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Description: Filter Tip; MultiRack Filter tips |
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| 4137 | CORNING | 960/pk | EUR 341 |
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Description: Filter Tip; Deckwork Filter Tips |
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| MRF-100-L-R-S | CORNING | 960/pk | EUR 357 |
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Description: Filter Tip; MultiRack Maxymum Recovery Filter tips |
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| 30211-100ul | SAB | 100ul | EUR 252 |
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| 30212-100ul | SAB | 100ul | EUR 252 |
 MonoMethyl-Histone H4-K5 pAb |
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| 30214-100ul | SAB | 100ul | EUR 252 |
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| 30219-100ul | SAB | 100ul | EUR 252 |
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| 30221-100ul | SAB | 100ul | EUR 252 |
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| 30222-100ul | SAB | 100ul | EUR 252 |
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| 30223-100ul | SAB | 100ul | EUR 252 |
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| 30225-100ul | SAB | 100ul | EUR 252 |
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| 30226-100ul | SAB | 100ul | EUR 252 |
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| 30227-100ul | SAB | 100ul | EUR 252 |
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| 30228-100ul | SAB | 100ul | EUR 252 |
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| 30229-100ul | SAB | 100ul | EUR 252 |
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| 30230-100ul | SAB | 100ul | EUR 252 |
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| 30231-100ul | SAB | 100ul | EUR 252 |
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| 30232-100ul | SAB | 100ul | EUR 252 |
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| 30233-100ul | SAB | 100ul | EUR 252 |
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| 30234-100ul | SAB | 100ul | EUR 252 |
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| 30235-100ul | SAB | 100ul | EUR 252 |
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| 30236-100ul | SAB | 100ul | EUR 252 |
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| 30238-100ul | SAB | 100ul | EUR 252 |
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| 30240-100ul | SAB | 100ul | EUR 252 |
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| 30241-100ul | SAB | 100ul | EUR 252 |
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| 30242-100ul | SAB | 100ul | EUR 252 |
 FoxO1/FoxO3a/FoxO4 Polyclonal Antibody |
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| 30243-100ul | SAB | 100ul | EUR 252 |
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| 30244-100ul | SAB | 100ul | EUR 252 |
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| 30245-100ul | SAB | 100ul | EUR 252 |
 C10orf67 Polyclonal Antibody |
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| 30246-100ul | SAB | 100ul | EUR 252 |
 LAPTM5 Polyclonal Antibody |
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| 30247-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] BAK1 Polyclonal Antibody |
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| 30248-100ul | SAB | 100ul | EUR 252 |
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| 30249-100ul | SAB | 100ul | EUR 252 |
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| 30251-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] IRF9 Polyclonal Antibody |
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| 30252-100ul | SAB | 100ul | EUR 252 |
 ACP1 Polyclonal Antibody |
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| 30253-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] AKR1A1 Polyclonal Antibody |
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| 30255-100ul | SAB | 100ul | EUR 252 |
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| 30256-100ul | SAB | 100ul | EUR 252 |
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| 30257-100ul | SAB | 100ul | EUR 252 |
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| 30258-100ul | SAB | 100ul | EUR 252 |
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| 30259-100ul | SAB | 100ul | EUR 252 |
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| 30261-100ul | SAB | 100ul | EUR 252 |
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| 30262-100ul | SAB | 100ul | EUR 252 |
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| 30263-100ul | SAB | 100ul | EUR 252 |
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| 30264-100ul | SAB | 100ul | EUR 252 |
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| 30265-100ul | SAB | 100ul | EUR 252 |
[KO Validated] EMD Polyclonal Antibody |
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| 30266-100ul | SAB | 100ul | EUR 252 |
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| 30267-100ul | SAB | 100ul | EUR 252 |
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| 30268-100ul | SAB | 100ul | EUR 252 |
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| 30269-100ul | SAB | 100ul | EUR 252 |
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| 30270-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] CTTN Polyclonal Antibody |
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| 30271-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] LGALS1 Polyclonal Antibody |
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| 30272-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] CTNND1 Polyclonal Antibody |
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| 30273-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] FADD Polyclonal Antibody |
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| 30274-100ul | SAB | 100ul | EUR 252 |
 CYP24A1 Polyclonal Antibody |
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| 30275-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] LIG4 Polyclonal Antibody |
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| 30276-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] FTL Polyclonal Antibody |
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| 30277-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] PTBP1 Polyclonal Antibody |
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| 30278-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] BUB1 Polyclonal Antibody |
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| 30279-100ul | SAB | 100ul | EUR 252 |
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| 30280-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] NDRG1 Polyclonal Antibody |
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| 30281-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] NFKB2 Polyclonal Antibody |
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| 30282-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] UBE2S Polyclonal Antibody |
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| 30283-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] NLRX1 Polyclonal Antibody |
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| 30284-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] ERCC1 Polyclonal Antibody |
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| 30285-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] DBI Polyclonal Antibody |
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| 30286-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] MPG Polyclonal Antibody |
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| 30287-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] NDUFA13 Polyclonal Antibody |
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| 30288-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] CBR1 Polyclonal Antibody |
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| 30289-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] UBE2C Polyclonal Antibody |
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| 30290-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] SMAD4 Polyclonal Antibody |
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| 30291-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] BMPR2 Polyclonal Antibody |
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| 30292-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] DIAPH1 Polyclonal Antibody |
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| 30293-100ul | SAB | 100ul | EUR 252 |
[KO Validated] FADD Polyclonal Antibody |
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| 30294-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] TFRC Polyclonal Antibody |
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| 30295-100ul | SAB | 100ul | EUR 252 |
[KO Validated] PTBP1 Polyclonal Antibody |
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| 30296-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] COMT Polyclonal Antibody |
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| 30297-100ul | SAB | 100ul | EUR 252 |
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| 30298-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] HNRNPA1 Polyclonal Antibody |
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| 30299-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] LDHB Polyclonal Antibody |
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| 30300-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] ATG4D Polyclonal Antibody |
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| 30301-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] ALDH9A1 Polyclonal Antibody |
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| 30302-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] STK4 Polyclonal Antibody |
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| 30303-100ul | SAB | 100ul | EUR 252 |
[KO Validated] PSIP1 Polyclonal Antibody |
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| 30304-100ul | SAB | 100ul | EUR 252 |
 [KO Validated] MYL6B Polyclonal Antibody |
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| 30305-100ul | SAB | 100ul | EUR 252 |
 K6-linkageSpecificPolyubiquitin Polyclonal Antibody |
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| 30306-100ul | SAB | 100ul | EUR 252 |
 FNDC5 Polyclonal Antibody |
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| 30307-100ul | SAB | 100ul | EUR 252 |
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| 30308-100ul | SAB | 100ul | EUR 252 |
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| 30309-100ul | SAB | 100ul | EUR 252 |
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| 30310-100ul | SAB | 100ul | EUR 252 |
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| 30311-100ul | SAB | 100ul | EUR 252 |
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| 30312-100ul | SAB | 100ul | EUR 252 |
 MEK1/MEK2 Polyclonal Antibody |
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| 30313-100ul | SAB | 100ul | EUR 252 |
 TEX101 Polyclonal Antibody |
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| 30314-100ul | SAB | 100ul | EUR 252 |
 RXRB Polyclonal Antibody |
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| 30315-100ul | SAB | 100ul | EUR 252 |
 FBXW8 Polyclonal Antibody |
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| 30316-100ul | SAB | 100ul | EUR 252 |
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| 30317-100ul | SAB | 100ul | EUR 252 |
 HIS-O2 Polyclonal Antibody |
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| 30318-100ul | SAB | 100ul | EUR 252 |
 PFK Polyclonal Antibody |
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| 30319-100ul | SAB | 100ul | EUR 252 |
 BDNF Monoclonal Antibody |
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| 30320-100ul | SAB | 100ul | EUR 252 |
 PTPRO Polyclonal Antibody |
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| 30321-100ul | SAB | 100ul | EUR 252 |
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| 30322-100ul | SAB | 100ul | EUR 252 |
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| 30323-100ul | SAB | 100ul | EUR 252 |
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| 30324-100ul | SAB | 100ul | EUR 252 |
 PML（isoform 5） Polyclonal Antibody |
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| 30325-100ul | SAB | 100ul | EUR 252 |
 E-Cadherin Monoclonal Antibody |
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| 30326-100ul | SAB | 100ul | EUR 252 |
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| 30327-100ul | SAB | 100ul | EUR 252 |
 LYST Polyclonal Antibody |
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| 30328-100ul | SAB | 100ul | EUR 252 |
 TBCCD1 Polyclonal Antibody |
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| 30329-100ul | SAB | 100ul | EUR 252 |
 ED1 Polyclonal Antibody |
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| 30330-100ul | SAB | 100ul | EUR 252 |
 OSBPL5 Polyclonal Antibody |
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| 30331-100ul | SAB | 100ul | EUR 252 |
 CYSLTR1 Polyclonal Antibody |
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| 30332-100ul | SAB | 100ul | EUR 252 |
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| 30333-100ul | SAB | 100ul | EUR 252 |
 SHFM1 Polyclonal Antibody |
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| 30334-100ul | SAB | 100ul | EUR 252 |
 AJUBA Polyclonal Antibody |
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| 30335-100ul | SAB | 100ul | EUR 252 |
Including post-translational modification reactions involving these multivalent proteins can account for the fast dynamics of the ensuing clusters, as is observed via microscopy and photobleaching experiments. The clusters found in simulations also give rise to chromatin domains that conform well with the observation of A/B compartments in HiC experiments.